Essay on Serial Dilutions - 616 Words

Serial Dilution Activity Many applications require the determination of microbial numbers. Those applications can be either clinical or in a research setting. Clinical applications include determination of antibiotic efficacy and as well as therapy. Research applications include determination of the effectiveness of antimicrobial chemicals, radiation, etc. The viable count is most common or standard method used to quantitate bacteria. With this method only microbes that are alive and able to reproduce can be counted. Since microbes grow into such large numbers it is extremely difficult to perform this without diluting the microbial culture first. A key concept here is the ability of microbes such as bacteria and fungi to form visible†¦show more content†¦I would take test tubes of different concentrations of the bacterial samples put one sample of each on a petri dish; diluting the large amount of bacteria to different concentrations. A dilution of 200 X 10^-6 = 2x 10^8 cfu/ml. You would go about finding this out by taking four other test tubes adding 1.0 ml of to one of the test tubes and shaking them. I would use a general wide series of dilutions ranging from 10^2 to 10^8. After shaking this test tube #1 with 10^8 dilution, you would take a pipet and transfer 1.0 ml aseptically to the next test tube of 10^6. Shake the test tube of 10^6 dilution and aseptically transfer 1.0 ml to the test tube of 10^4 dilutions. Shake the test tube of 10^4 dilution and aseptically transfer 1.0 ml to the test tube of 10^2 dilution with a pipet. Now shake the test tube of 10^8and take a new pipet transfer 1.0 ml to one petri dish and 0.1 ml to another petri dish. Do this same task to test tubes 10^6 and 10^4. I would then go about mixing a agar with the 10^8. I would do this to the remaining plates and allow the agar time to cool and harden. When the plates have hardened I would then go about counting the plates that have between 25-250 colonies. This how I would go about double checking the accuracy of the concentration of thisShow MoreRelatedEvaluation Of A Chemical Substance1561 Words   |  7 Pagesspectrophotometer. This device consists of a spectrometer that produces light of any color (wavelength), and a photometer which measures the intensity of the light.2 In this experiment serial dilutions will be performed and from this, calibration curves will be produced. New solutions will be created from these dilutions and each new concentration will be found by using the concentration and volume formula given in the manual. In addition, Beer’s Law will be utilized to determine the concentrations ofRead MoreSpectrophotometer: Concentration and Standard Curve Essay805 Words   |  4 Pagesspectrum to determine the concentration of light absorbing molecules in a solution. (p.59) In this particular lab, our mission was to determine the protein concentration and the standard curve of the unknown sample of BSA. This, by preparing five dilutions of the unknown solution of BSA together with other known concentrations, and then experimenting by observing how the concentrations were passed through the spectrophotometer. The outcome resolved in the absorption levels being decreased, and thisRead MoreEssay about Micro Lab Project757 Words   |  4 PagesProfessor Rotibi ABSTRACT: Accurate evaluation of bacterial colonization as a predictive index for alfalfa sprouts has relied on a quantitative culture technique that provides exact colony counts per gram of tissue by culture of five serial dilutions of the alfalfa water. In this study 1 package of alfalfa sprouts were cultured by a semi-quantitative technique that enumerated the number of gram-negative enteric organism in 1 ml of alfalfa water. Exact colony counts from the experiment wereRead MoreBacterial Enumeration of Various Meat Products1161 Words   |  5 Pagescurrently collected samples of hamburger, suspension were transferred via serial dilution, and then transferred onto spread plates and pour plates. The samples were permitted to culture for approximately one week, and then enumerated through use of colony counting methods. Although, methods were taken to transfer each sample to their dilutions aseptically, any contamination may result in skewed results; however, the dilution number will directl y influence the number of bacterial colonies that formRead MoreThe Isolation Of Milk Whey904 Words   |  4 Pagesset of five serial dilutions of BSA and the purified samples were made as shown in Table * and *, respectively. Table 1. BSA standard serial dilutions. Cuvette Serial Additions 0.01% SDS Volume Dilution Factor 1 50  µL of 1 mg/mL BSA 950  µL 50  µg/mL 2 500  µL of #1 500  µL 25  µg/mL 3 500  µL of #2 500  µL 12.5  µg/mL 4 500  µL of #3 500  µL 6.25  µg/mL 5 500  µL of #4 500  µL 3.125  µg/mL 6 Discard 500  µL of #5 500  µL 0  µg/mL Table 2. Serial dilutions of purified ÃŽ ±-lactalbumin. Cuvette Serial Additions 0Read MoreLab Investigation : Cell Purple Dye Solution1042 Words   |  5 Pagesto the guiding question of: how to make 1 L of Pirate Purple dye. Section 2 Performing this lab, a serial dilution was set up for the red and blue dyes. After labeling 5 beakers A-D and a Stock of 30 mL of the dye solution, 10 mL of the Stock solution plus 10 mL of water was transferred into beaker A; then 10 mL of beaker A plus 10 mL of water into beaker B and so forth. After the serial dilutions of the red and blue dyes were taken, the molarity and absorbance for both dyes were calculated. UsingRead MoreQuestions On The Quantification Of Light Absorption And Transmittance As A Function Of A Solution1804 Words   |  8 Pagesthe procedure involved serial dilution. In other words, 50% of the original sample was transferred to container number two and diluted to the mark. This step was repeated with the rest of the containers; the idea was to reduce the concentration of each container by a factor of  ½. The unknown was dissolved as instructed in the label and the absorbances of four trials were obtained, to account for a 95% certainty on the concentrations reported. Sample of serial dilutions in Figure 2. (VolumesRead MoreAntibacterial Effects Of The Chloramphenicol Antibiotic On Escherichia Coli1473 Words   |  6 Pagesrange of 25ÃŽ ¼g/mL and 170ÃŽ ¼g/mL. Methods and Materials Our study took place in a laboratory in Duncan Hall at San Jose State University. The chemistry department of San Jose State University supplied our materials. We conducted serial dilutions and calculated our dilution factors using the equation: C1V1=C2V2 The study resulted in a series of agar plates containing E. coli and Chloramphenicol. The plates had progressively higher antibiotic concentrations (from no antibiotic on one end and excessRead MoreGrowth Curve Report1680 Words   |  7 Pagesgrowth of yeast is measured by using spectrophotometer and hemocytometer.We learnt how specthophotometer and hemocytometer use and also we learnt qualifications of hemocytometer and spectrophotometer.Serial dilution was used for this experiment and it was very important.Because of the serial dilution,we measured the number of yeast cells. The graph of growth curve was drawn and bacterial life cycle was understood with the graph.The purpose of the experiment was to calculate and draw bacterial growthRead MoreEssay Quantitave Plating642 Words   |  3 Pagessee the effects of pasteurization while emphasizing the process for serial dilutions. PROCEDURE See references (1) RESULTS As the dilution factor increased for both the raw milk (unpasteurized) and pasteurized milk samples, the number of colonies decreased. The number of cells/mL in the pasteurized milk sample is considerably less than the number of cells/mL in the raw milk sample. RAW (UNPASTEURIZED) SAMPLE Dilution Factor | Number of Colonies | Number of cells/mL | 10-3 | TMTC |

Sentimental and Frontier Adventure in The Last of the Mohicans Movie Review

Essays on Sentimental and Frontier Adventure in The Last of the Mohicans Movie Movie Review The paper "Sentimental and Frontier Adventure in The Last of the Mohicans Movie" is a good example of a movie review on visual arts and film studies. The film is a very interesting historical film. It was set up during the French and Indian War in 1757. During this war, the French called on allied Native American tribes to battle with the tough British in the region (Cooper, 24). Apart from the plot, the film offers many themes and the most interesting question that arises is: How does The Last of the Mohicans draw together elements of the syrupy novel and the frontier adventure story? Fact oneCooper incorporates and entangles elements of the sentimental novel, such as marriage and love and sentiments of the frontier adventure, such as racial conflict and warfare. Cooper develops psychological tensions and friendship among the characters that are basically of both genres.Fact twoApart from friendship and psychological tensions, Cooper utilizes female characters to establish the narrative weight of sentimentality. In addition, he introduced these women characters into the struggling state of affairs that define the frontier venture. In this film; Cooper creates a warfare environment characterized by emotions and dramas, through permeating it with syrupy elements of romance (Cooper, 65). Fact threeCooper heightens the film’s drama by demonstrating the Indian culture as good, Uncas, as opposed to the Indian figure of evil, Magua, as revealed in the contest for the love of Cora Munro-the white girl (Cooper, 45). He uses Magua and Uncas’s interracial aspir ations, so different in tone and intent, to incorporate psychological depth to the or else simple antagonism that exists between Indian and white. Conclusion While Cooper sees the elements of civilization as important, he feels that there is a trace of savagery in all humanity. He, therefore, develops a hybrid genre, frontier romance, through linking war and sentimental (Cooper, 105).

Introduction to Ancient Fine Arts Essay Example For Students

Introduction to Ancient Fine Arts Essay Painting and Other forms Of work Of art were patronized and practiced at the Kings and rulers palaces. Only their palaces were made of bricks and pillars of stone. General peoples houses were made of long grass, hay, clay and bamboo. This area was ruled by the Murray dynasty before the birth of Christ. The most prominent king of this dynasty was the king Shook. The emperors of Guppy Dynasty ruled from 400 AD for a few centuries. The most famous kings of Guppy Dynasty were King Samara Guppy, Kumar Guppy, Banyan Guppy foremost, Afterwards The Emperors of Pal Dynasty ruled for a few entries. The most dominant emperors of Pal Dynasty were Copal, Dharma, Devil, Mobile, Ramp foremost, After them gorgon and Seen Dynasty came to rule. At that time the Bengal was divided into two. North and north-western areas name was Pandora-Fordham Gourd Kingdom. The name of south-eastern part was Smooth or Plain land. In the year 1204 AD. Bookstore Kill deposed the king Alaskan Seen and started to rule Bengal He was Turkey by born, After that a few centuries were ruled by Muslim emperors before the regime of king Caber_ That time is known as Sultana regime. The most prominent Sultans of his time were Foxhounds Embark Shah, Shinnied Alias Shah, Sander Shah, Standing Lam Shah, Jaundice Mohammad Shah, Aladdin Hosanna Shah, Standing Manhood foremost. After them Mussels ruled Bengal, in between some independent Kings. The last independent king Of Bengal, king Syria-du-dual was deposed from the throne and was killed and British regime began. After NON centuries Of British regime, in 1947 two countries named as India and Pakistan became liberated. Pakistan had two parts. East Pakistan and West Pakistan. In 1971 we fought against the West Pakistanis Army and vibrated East Pakistan as present Bangladesh. Since last few thousands of years Bangle was ruled as a whole land or divided anatomical lands by different kings or rulers. There was always rivalry and war against one region to another. It was very normal to do robbery; burning down, and destroying was very normal. For this reason many areas used to become ruined or depopulated. After staying desolate for long time it used to get buried under earth. Because tot natural disasters like cyclones, earthquakes, it used to go under earth too, After excavation tot lumps tot earth or digging long ditches in Bangladesh ,three ruins f human settlements were found like, These are in Rajahs areas Paragraph, Somalias Antimony, Misanthrope. Many things got destroyed for staying under earth for long time. A few things have been found some weapons made of iron and copper, crockery, Statue, broken earthenware, terracotta plaques, statue of stone, stone inscription, royal decree, arch, Pillar, broken structures of buildings and houses, contemporary coins and ornaments of that time. All these findings are some testimony Of ancient fine arts and architecture. We can understand the patterns of ancient architecture from the ruins of buildings and souses, pillars, arches, and walls. Paintings and Other works Of art made from soft material is impossible to survive under earth and in natural calamities. The only things those survived are decaying iron, copper, gold, silver, stone and some terracotta goods. Which is already earlier mentioned that they are preserved in different museums. The most prominent among ancient works of art of Bangladesh is statue made of osteopaths or black stone. These famous statues can be observed in the National Museum in Dacha and in Bored Museum, Terracotta plaques, Stone engraved nameplates, and stone inscriptions re famous ancient work of art. Those which were found in archaeological excavation are now placed in different museums. Those type of traditional slabs are still on many old buildings, on the walls of mosques, and temples. The famous buildings with this type of plaques are Rajahs5 Shot Shown Mosque, Bag mosque, Assume mosque,Patria Raisers Mosque, Chula Backchats Seat Gumbo mosque (Sixty domed mosque), and Khan Johan Ala 5 token of ancient architecture are also famous. .ud2cd42aae9f8988f07c5e761fca4d147 , .ud2cd42aae9f8988f07c5e761fca4d147 .postImageUrl , .ud2cd42aae9f8988f07c5e761fca4d147 .centered-text-area { min-height: 80px; position: relative; } .ud2cd42aae9f8988f07c5e761fca4d147 , .ud2cd42aae9f8988f07c5e761fca4d147:hover , .ud2cd42aae9f8988f07c5e761fca4d147:visited , .ud2cd42aae9f8988f07c5e761fca4d147:active { border:0!important; } .ud2cd42aae9f8988f07c5e761fca4d147 .clearfix:after { content: ""; display: table; clear: both; } .ud2cd42aae9f8988f07c5e761fca4d147 { display: block; transition: background-color 250ms; webkit-transition: background-color 250ms; width: 100%; opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #95A5A6; } .ud2cd42aae9f8988f07c5e761fca4d147:active , .ud2cd42aae9f8988f07c5e761fca4d147:hover { opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #2C3E50; } .ud2cd42aae9f8988f07c5e761fca4d147 .centered-text-area { width: 100%; position: relative ; } .ud2cd42aae9f8988f07c5e761fca4d147 .ctaText { border-bottom: 0 solid #fff; color: #2980B9; font-size: 16px; font-weight: bold; margin: 0; padding: 0; text-decoration: underline; } .ud2cd42aae9f8988f07c5e761fca4d147 .postTitle { color: #FFFFFF; font-size: 16px; font-weight: 600; margin: 0; padding: 0; width: 100%; } .ud2cd42aae9f8988f07c5e761fca4d147 .ctaButton { background-color: #7F8C8D!important; color: #2980B9; border: none; border-radius: 3px; box-shadow: none; font-size: 14px; font-weight: bold; line-height: 26px; moz-border-radius: 3px; text-align: center; text-decoration: none; text-shadow: none; width: 80px; min-height: 80px; background: url(https://artscolumbia.org/wp-content/plugins/intelly-related-posts/assets/images/simple-arrow.png)no-repeat; position: absolute; right: 0; top: 0; } .ud2cd42aae9f8988f07c5e761fca4d147:hover .ctaButton { background-color: #34495E!important; } .ud2cd42aae9f8988f07c5e761fca4d147 .centered-text { display: table; height: 80px; padding-left : 18px; top: 0; } .ud2cd42aae9f8988f07c5e761fca4d147 .ud2cd42aae9f8988f07c5e761fca4d147-content { display: table-cell; margin: 0; padding: 0; padding-right: 108px; position: relative; vertical-align: middle; width: 100%; } .ud2cd42aae9f8988f07c5e761fca4d147:after { content: ""; display: block; clear: both; } READ: Some Famous Tapestries EssayThere are more illustration of this type of architecture-Dachas Boor Extra,Shot Extra, Alba forte, Pià ±ata Bibs mosque, Khan Mohammad Marinas mosque, Dishware temple, Tara mosque, Susan Mongol, Shutterbug mosque, Haggling forte of Narration, Assonants forte, Antiradar forte of Nonsingular Bangle temple of Pan, Gear Sinned said mosque of Enmeshing, Shah Mohammad mosque, Shallots Major Of Sylphs, Major of Shah Apron, Megalith stone of Genitourinary, Somalias Shooter Rota temple, Biased Bobstays Major Of Chitchatting, and many more buildings and places. Arts and Crafts in Living From ancient time many people have dependent their living on fine arts and crafts. They have chosen work of art as their profession. Our folk Artists and crafts men are fulfilling their necessities of life by the money they receive from selling their work of art. For example fancy items like terracotta dolls, fancy crockery, wooden dolls with designs, elephant, horse, Mackintosh of village women etc. Are collected by people in exchange of money since long time ago. Reticulate bag made of jute, different types of bags made of jute are produced, tablemate and many other things are also commercially successful. Earthen potteries of the potters, besides pitchers, many other crafts are produced. For example small and huge flower vase, earthen sculpture, birds made of clay etc. Wood, bamboo and cane are used to make different turpitude, musical instrument, to portray picture and design; many artists of fine arts and crafts are working on it. Arts and Crafts 3 Handloom saris of Tangling of Bangladesh are famous at home and abroad for its color, design and texture. Efficient craftsmen weave Sandmans decorative design with colorful threads. Decorative handloom saris are not highly demanded at home only but also by people who live abroad, collect it with great interest, For example Jamaican, Dakar 8TH, Tangling Sari and the aborigine peoples decorative colorful handloom dresses. In Bangladesh there are many educational institutions of Arts and Crafts. Many of them are connected with universities, besides, there are many private institutions too. Every year more than four hundred artists passes out from these educational institutions. They contribute in fine arts and crafts in different organizations Of the country. For example in advertising farms, drawing pictures for books, newspaper, cinema industry, starting form building sets for television to different work Of art, garments industry, pharmaceutical industries and factories, including interior design of many other buildings our artists of Arts and Crafts have designed and completed successfully.

Obstacles to National Unity free essay sample

The obstacles to national unity Racial Inequality, Social Inequality, Religious inequality etc (1) Regionalism/ Separatism (it) Linguism (iii) Casteism and (v) communalism. (i) Regionalism/Separatism: Regionalism or Separatism is the most advance obstacle in the way of National Unity and development. Above all regionalism and separatism are threatening to balkanize the country. Aggressive regionalism has gravely undermined the feeling of unity of the people. It creates a parochial outlook and narrow-mindedness. It is primarily a socio economic problem, related to the removal f the obstacles in the development of the people according to their genius and culture. The extremely uneven economic development of the different regions of India has created tensions and Jealousy between the states as to which should get priority in the matter of new projects and industries. These tensions have their origin in genuine grievances of the regions and states that have been denied fair shares of projects and industries in the overall structure of development. We will write a custom essay sample on Obstacles to National Unity or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page The only way to do away with this imbalance in the development is to reduce and eliminate these disparities gradually. The importance of regional balance in economic development and social security as the positive factors for promoting national unity as well as in integration. Therefore, the rapid development of economically backward regions in any state should be given priority in national and state plans at least to the extent that a minimum level of development is reached for all states within a framed period. While this problem is not only an economic one, there is no doubt that a rapid and balanced economic development calculated to wipe out regional disparities would go a long way towards promoting national unity. Indias unity in the midst of diversity was stressed. (it) Linguism: India is a multi lingual country. The language controversy started with Hindi being given the status of the official language of the Indian union. The people of the south revolted against it and threatened to go out of the Union if Hindi was imposed upon them. The constitution of India was amended to meet their demand. The plea of the south is that recognition of Hindi as the official language will be implication and in effect create conditions for the domination of one linguistic group over all others. The language issue is very sensitive one. We must not forget how passions were aroused on minor issues concerning reorganization of states on the linguistic basis. The linguistic fanaticism is had, but it has got to be tamed with love, understanding and accommodation not by adopting rigid attitudes and postures. Some solution to the problem which may be acceptable to all linguistic groups must be solved and evolved. Imposition of any language by force will only worsen the situation accordingly. (iii)Casteism : Casteism is another evil that stands in the way of complete national unity. The most shameful feature of the caste system is the existence untouchability,